a300 587a Search Results


anti nono antibodies  (Bethyl)
93
Bethyl anti nono antibodies
(a,b) Heatmap and volcano plot show differentially expressed genes in shLnc473 knockdown in compared with shRNA control in fusion-positive H3118 cells. The cutoff criteria were absolute fold-change of ≥2 and p<0.05. This analysis led to the identification of a total of 1320 LINC00473-regulated candidate coding genes, with 675 up-regulated and 645 down-regulated genes. (c) Functional classification of LINC00473 down-regulated genes was performed using Ingenuity Pathway Analysis (IPA). The top 6 molecular and cell functions were ranked based on p-value and activation z-score. Negative z-score indicates inhibition. (d) The qRT-PCR analysis showed that LncRNA LINC00473 was significantly enriched in the <t>NONO</t> immunoprecipitates relative to <t>the</t> <t>IgG</t> control in H3118 MEC cells. ASNS was used as a negative control (n=3, ***p<0.0001). (e) Overexpression of LINC00473 in HEK293T cells significantly increased the interaction of CRTC1-MAML2 and NONO by a Gal4-NONO reporter assay (n=3, *p<0.05). (f) Knockdown of NONO or LINC00473 expression significantly reduced the pCRE-luc reporter activities in HEK293T cells with CRTC1-MAML2 overexpression (n=3, *p<0.05 and **p<0.001). (g) A model for the molecular basis of LINC00473 induction by CRTC1-MAML2 and function in fusion-positive MEC cells.
Anti Nono Antibodies, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a300+587a/NONO+Antibody/pmc05889358-168-10-13
Average 93 stars, based on 1 article reviews
anti nono antibodies - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results


(a,b) Heatmap and volcano plot show differentially expressed genes in shLnc473 knockdown in compared with shRNA control in fusion-positive H3118 cells. The cutoff criteria were absolute fold-change of ≥2 and p<0.05. This analysis led to the identification of a total of 1320 LINC00473-regulated candidate coding genes, with 675 up-regulated and 645 down-regulated genes. (c) Functional classification of LINC00473 down-regulated genes was performed using Ingenuity Pathway Analysis (IPA). The top 6 molecular and cell functions were ranked based on p-value and activation z-score. Negative z-score indicates inhibition. (d) The qRT-PCR analysis showed that LncRNA LINC00473 was significantly enriched in the NONO immunoprecipitates relative to the IgG control in H3118 MEC cells. ASNS was used as a negative control (n=3, ***p<0.0001). (e) Overexpression of LINC00473 in HEK293T cells significantly increased the interaction of CRTC1-MAML2 and NONO by a Gal4-NONO reporter assay (n=3, *p<0.05). (f) Knockdown of NONO or LINC00473 expression significantly reduced the pCRE-luc reporter activities in HEK293T cells with CRTC1-MAML2 overexpression (n=3, *p<0.05 and **p<0.001). (g) A model for the molecular basis of LINC00473 induction by CRTC1-MAML2 and function in fusion-positive MEC cells.

Journal: Oncogene

Article Title: CRTC1-MAML2 fusion-induced lncRNA LINC00473 expression maintains the growth and survival of human mucoepidermoid carcinoma cells

doi: 10.1038/s41388-017-0104-0

Figure Lengend Snippet: (a,b) Heatmap and volcano plot show differentially expressed genes in shLnc473 knockdown in compared with shRNA control in fusion-positive H3118 cells. The cutoff criteria were absolute fold-change of ≥2 and p<0.05. This analysis led to the identification of a total of 1320 LINC00473-regulated candidate coding genes, with 675 up-regulated and 645 down-regulated genes. (c) Functional classification of LINC00473 down-regulated genes was performed using Ingenuity Pathway Analysis (IPA). The top 6 molecular and cell functions were ranked based on p-value and activation z-score. Negative z-score indicates inhibition. (d) The qRT-PCR analysis showed that LncRNA LINC00473 was significantly enriched in the NONO immunoprecipitates relative to the IgG control in H3118 MEC cells. ASNS was used as a negative control (n=3, ***p<0.0001). (e) Overexpression of LINC00473 in HEK293T cells significantly increased the interaction of CRTC1-MAML2 and NONO by a Gal4-NONO reporter assay (n=3, *p<0.05). (f) Knockdown of NONO or LINC00473 expression significantly reduced the pCRE-luc reporter activities in HEK293T cells with CRTC1-MAML2 overexpression (n=3, *p<0.05 and **p<0.001). (g) A model for the molecular basis of LINC00473 induction by CRTC1-MAML2 and function in fusion-positive MEC cells.

Article Snippet: Cell lysates were collected for immunoprecipitation overnight at 4°C using anti-NONO antibodies (A300-587A, Bethyl Laboratories) control and IgG control (SC-2027, Santa Cruz) and protein A/G beads.

Techniques: Knockdown, shRNA, Control, Functional Assay, Activation Assay, Inhibition, Quantitative RT-PCR, Negative Control, Over Expression, Reporter Assay, Expressing